Troubleshooting

Problem: Growth on the Slant within First Week of Incubation

Possible causes Solutions

1. No proper decontamination of sputum Proper decontamination of sputum specimen is to be carried out using Lyfectol

specimen due to which contaminants (decontamination reagent) to ensure that all unwanted organisms are killed

over grow

2. Fast growing organism of Mycobacterium Confirm the results with biochemical tests

species

Microbiology and Bacteriology 857

Problem: Collapse of Slants

Possible causes Solutions

1. Improper transportation, i.e. kits subjected Discard the slant and use fresh slant

to violent jerks and vibrations or not

handled with care

2. Contaminated slants If the slants are found contaminated because of various reasons, discard the slants and use

fresh slants to perform the test

3. Contaminated untreated sputum sample Pretreat sputum sample prior to inoculation

used

COMBIPACK OF SOLID AND LIQUID MEDIUM

FOR MYCOBACTERIUM TUBERCULOSIS

ISOLATION

(Courtesy: Tulip Group of Companies)

Combicult®