1. Discolored, dislodged, or contaminated medium

should not be used.

2. Ensure that clean catch midstream urine samples are

used so that surrounding external microbial flora do

not give discrepant results.

3. Observe aseptic conditions while performing the

test to avoid contamination with non-pathogenic

bacteria.

4. Urine specimens should be collected and cultured

immediately. Prolonged storage of urine samples

at room temperature (25–30oC) may result in

multiplication of contaminating organisms and raise

the bacterial count leading to discrepancies.

5. Cultures of three consecutive first morning urine

specimens are universally recommended since the

reliability of the test increases to approximately 100%.

6. Whenever possible indicate if the patient is on

antibiotic therapy, since urine samples of patients on

antibiotic therapy may not show bacterial growth or

may have low colony count.

7. Treat the specimens and used slants by immersing in

2% activated glutaraldehyde for at least 2 hours before

incineration and disposal.

8. For confirmation and identification of the isolated

organism, it is recommended to perform gram stain,

biochemical or serological studies.

9. Good laboratory practices and hazard precautions

must be observed at all times.

10. When performed correctly, EASYBACT results correlate

with standard plate method using calibrated loop.

11. Optionally calibrated loops can also be used for

inoculating the sample.

Notes

Procedure for collection of clean catch midstream urine

samples. The objective is to collect a specimen, which will

reflect as much as possible only the urine present in the

urinary bladder.