If the patients can be explained clearly, EASYBACT vial

may be used to directly collect the midstream clean catch

samples (Refer Notes for collection of midstream clean

catch urine).

Test Procedure

1. Retrieve the required number of EASYBACT vials from

the carton.

2. Bring the slants to room temperature (25–30oC) prior

to testing.

834 Concise Book of Medical Laboratory Technology: Methods and Interpretations

3. Label the EASYBACT vials appropriately with the

patient’s ID.

4. Open the EASYBACT vial observing aseptic conditions.

5. Directly collect clean catch midstream urine into

the EASYBACT vial or add urine samples from

catheterization/suprapubic taps into the EASYBACT

vial, right up to the brim.

6. Empty the urine sample from the EASYBACT vial

immediately, observing aseptic conditions.

7. In case small amount of urine is retained in the vial,

drain the excess urine by gently tapping the mouth of

the EASYBACT vial on to a fresh clean blotting/filter

paper. This is done to ensure no excess urine remains

on the slant/in the bottle.

8. Recap the vial immediately.

9. Incubate the vial in an incubator, preset at 37oC for 18

to 24 hours, in an inverted position with the cap facing

downwards.

10. Read the results at the end of the incubation period.

Interpretation of Results

Density Count: Read the density of the colonies as per the

chart given below:

Identification of Bacteria

The pathogenic organisms can be identified as per the

color chart provided with the EASYBACT kit and the table

mentioned below.

Remarks