2. Make one light sweep through the lower portion of this

streaked area. Turn the plate at right angles and streak

approximately 1/2 of the remaining portion without

overlapping previous streaks.

3. Turn the plate and streak the remainder of the plate

avoiding previously streaked area.

4. The appearance of this plate will resemble that

obtained in method II.

Precautionary Measures to be Undertaken in a

Microbiology Laboratory

The infected material obtained from patients is to be treated

with utmost care. In fact, all individuals working in such a

laboratory should wear laboratory coats, wear gloves, use

nose and mouth masks and be immunized regularly for

prophylaxis against communicable diseases. Outlined

below are the rules, which must be observed.

1. Have an air pressure flow (air curtain) at the entry and

exit doors of the laboratory. Being expensive, if this

cannot be installed, the other precautions mentioned

below are to be rigidly followed.

2. Change clothing before entering and leaving the

laboratory. If this is not possible, use a laboratory

apron to be worn over the outdoor clothing.

3. After working with cultures, and always before one

leaves the laboratory, hands should be well washed,

first in some disinfectant solution and then with soap

and water.