Method II

This method of streaking may be used either for culture

from solid media or for heavy broth cultures.

1. Select the wanted colony from a crowded plate or

pick up growth from a slant with a needle. Streak this

carefully on a restricted area of the plate. Flame the

needle and put it away.

2. With the sterile cool loop make one light sweep

through the needle inoculated area and streak with

832 Concise Book of Medical Laboratory Technology: Methods and Interpretations

close parallel strokes the top one-fourth of the plate

surface. Flame the loop and allow it to cool.

3. Turn the plate and streak the remainder of the plate

with the same loop. Avoiding any areas previously

streaked.

4. Discrete colonies should appear in the areas which

was last streaked.

Method III

This method constitutes a simple technique and is used for

liquid culture largely but it may also be used for culture

from solid media.

1. Place a loopful of inoculum near the periphery of the

plate and cover approximately 1/4 of the plate with

close parallel streaks. Flame the loop and allow it to

cool.