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12/29/23

Download Clinical Emergency Medicine pdf

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 Notice Medicine is an ever-changing science. As new research and clinical experience broaden our knowledge, changes in treatment and drug therapy are required. The authors and the publisher of this work have checked with sources believed to be reliable in their eff01ts to provide information that is complete and generally in accord with the standards accepted at the time of publication. However, in view of the possibility of human error or changes in medical sciences, neither the authors nor the publisher nor any other party who bas been involved in the preparation or publication of this work warrants that the information contained herein is in every respect accurate or complete, and they disclaim all responsibility for any errors or omissions or for the results obtained from use of the information contained in this work. Readers are encouraged to confirm the information contained herein with other sources. For example and in particular, readers are advised to check the product information sheet included in the package of each drug they plan to administer to be ce1tain that the information contained in this work is accurate and that changes have not been made in the recommended dose or in the contraindications for administration. This recommendation is of particular imp01tance in connection with new or infrequently used drugs. TERMS OF USE This is a copyrighted work and McGraw-Hill Education and its licensors reserve all rights in and to the work. Use of this work is subject to these terms. Except as permitted under the Copyright Act of 1976 and the right to store and retrieve one copy of the work, you may not decompile, disassemble, reverse engineer, reproduce, modify, create derivative works based upon, transmit, distribute, disseminate, sell, publish or sublicense the work or any part of it without McGraw-Hill Education's prior consent. You may use the work for your own noncommercial and personal use; any other use of the work is strictly prohibited. Your right to use the work may be terminated if you fail to comply with these terms. THE WORK IS PROVIDED "AS IS." McGRAW-HILL EDUCATION AND ITS LICENSORS MAKE NO GUARANTEES OR WARRANTIES AS TO THE ACCURACY, ADEQUACY OR COMPLETENESS OF OR RESULTS TO BE OBTAINED FROM USING THE WORK, INCLUDING ANY INFORMATION THAT CAN BE ACCESSED THROUGH THE WORK VIA HY PERLINK OR OTHERWISE, AND EXPRESSLY DISCLAIM ANY WARRANTY, EXPRESS OR IMPLIED, INCLUDING BUT NOT IMlTED TO IMPLIED WARRANTIES OF MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE. McGraw-Hill Education and its licensors do not warrant or guarantee that the functions contained in the work will meet your requirements or that its operation will be uninterrupted or error free. Neither McGraw-Hill Education nor its licensors shall be liable to you or anyone else for any inaccuracy, error or omission, regardless of cause, in the work or for any damages resulting therefrom. McGraw-Hill Education bas no responsibility for the content of any information accessed through the work. Under no circumstances shall McGraw-Hill Education and/or its licensors be liable for any indirect, incidental, special, punitive, consequential or similar damages that result from the use of or inability to use the work, even if any of them bas been advised of the possibility of such damages. This limitation of liability shall apply to any claim or cause whatsoever whether such claim or cause arises in contract, t01i or otherwise.

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Contents Contributors viii Preface XVI Acknowledgments xvii I. Common Procedures IV. Pulmonary Emergencies 1. Incision and Drainage 1 20. Dyspnea 84 2. Arterial Blood Gas 4 21. Asthma 89 3. Central Venous Access 8 22. Chronic Obstructive Pulmonary Disease 95 4. Procedural Sedation 13 23. Pneumonia 100 5. Lumbar Puncture 16 24. Pneumothorax 105 6. Laceration Repair 20 25. Pulmonary Embolism 108 7. Needle and Tube Thoracostomy 24 V. Abdominal Emergencies 8. Introduction to Emergency Ultrasonography 27 26. Acute Abdominal Pain 112 II. Resuscitation 27. Appendicitis 118 9. Emergency Medical Services 30 28. Acute Cholecystitis 121 1 o. Cardiopulmonary Arrest 33 29. Abdominal Aortic Aneurysm 125 11. Airway Management 37 30. Gastrointestinal Bleeding 128 12. Shock 42 31. Intestinal Obstruction 131 Ill. Cardiovascular Emergencies 32. Mesenteric Ischemia 135 13. Chest Pain 46 VI. Infectious Disease Emergencies 14. Acute Coronary Syndromes 50 33. Fever 138 15. Congestive Heart Failure 57 34. Sepsis 142 16. Dysrhythmias 63 35. Meningitis and Encephalitis 147 17. Aortic Dissection 70 36. Soft Tissue Infections 151 18. Hypertensive Emergencies 75 37. Human Immunodeficiency Virus 156 19. Syncope 80 38. Blood and Body Fluid Exposure 162 v CONTENTS VI I. Genitourinary Emergencies XI. Environmental Emergencies 39. Nephrolithiasis 166 61. Hypothermia 259 40. Urinary Tract Infections 170 62. Cold-Induced Tissue Injuries 263 41. Testicular Torsion 174 63. Heat-Related Illness 267 42. Penile Disorders 177 64. Drowning Incidents 270 VI II. Obstetrics/Gynecologic Emergencies 65. Envenomation 274 43. Vaginal Bleeding 181 XI I. Metabolic/Endocrine Emergencies 44. Vaginal Discharge 185 66. Diabetic Emergencies 280 45. Preeclampsia and Eclampsia 189 67. Potassium Disorders 284 46. Emergency Delivery 192 68. Thyroid Emergencies 288 IX. Pediatric Emergencies 69. Adrenal Emergencies 292 47. The Pediatric Patient 196 XI II. Hematologic/Oncologic Emergencies 48. Pediatric Fever 201 70. Oncologic Emergencies 295 49. Respiratory Distress 206 71. Sickle Cell Emergencies 299 50. Abdominal Pain 212 72. Transfusion Reactions 304 51. Dehydration 217 73. Anticoagulant Therapy and Its Complications 308 52. Otitis Media 221 XIV. HEENT Emergencies 53. Pharyngitis 225 7 4. Slit Lamp Examination 312 X. Toxicology 75. Red Eye 315 54. The Poisoned Patient 230 76. Acute Visual Loss 319 55. Toxic Alcohols 235 77. Epistaxis 324 56. Acetaminophen Toxicity 239 78. Dental Emergencies 327 57. Salicylate Toxicity 244 XV. Neurologic Emergencies 58. Carbon Monoxide Poisoning 247 79. Altered Mental Status 332 59. Digoxin 251 80. Headache 336 60. Cyclic Antidepressants 255 81. Dizziness 341 CONTENTS vii 82. Cerebrovascular Accident 347 92. Low Back Pain 403 83. Seizures and Status Epilepticus 353 93. Compartment Syndromes 407 XVI. Trauma 94. Septic Arthritis 410 84. Trauma Principles 358 95. Splinting 414 85. Head Injuries 363 XVI II. Dermatologic Emergencies 86. Cervical Spine Injuries 368 96. Life-Threatening Dermatoses 418 87. Thoracic Trauma 374 97. Allergic Reactions 423 88. Abdominal Trauma 381 XIX. Psychiatric Emergencies 89. Burns 387 98. Approach to the Psychiatric Patient 426 XVI I. Orthopedic Emergencies Index 429 90. Upper Extremity Injuries 391 91. Lower Extremity Injuries 397 Contributors Negean Afifi, DO Department of Emergency Medicine Cook County (Stroger) Hospital Chicago, Illinois Steven E. Aks, DO Director, The Toxikon Consortiwn Division of Toxicology, Department of Emergency Medicine Cook County (Stroger) Hospital Associate Professor Department of Emergency Medicine Rush Medical College Chicago, Illinois Amer Zia Aldeen, MD Assistant Professor Department of Emergency Medicine Northwestern University Feinberg School of Medicine Attending Physician Department of Emergency Medicine Northwestern Memorial Hospital Chicago, Illinois Kim L. Askew, MD Assistant Professor Department of Emergency Medicine Wake Forest University School of Medicine Winston-Salem, North Carolina John Bailitz, MD, RDMS Emergency Ultrasound Director Department of Emergency Medicine Cook County (Stroger) Hospital Assistant Professor of Emergency Medicine Department of Emergency Medicine Rush Medical College Chicago, Illinois Jeffery A. Baker, MD Attending Physician Department of Emergency Medicine Ochsner Health System Clinical Instructor University of Queensland, Ochsner Clinical School New Orleans, Louisiana viii Jonathan Bankoff, MD, FACEP Medical Director Emergency Department Middlesex Hospital Middletown, Connecticut Eric H. Beck, DO, NREMT-P Assistant Professor Section of Emergency Medicine The University of Chicago EMS Medical Director Chicago EMS Illinois EMS Region 1 1, Medical Directors Consortium Chicago, Illinois Lauren Emily Bence, MD Department of Emergency Medicine University of Chicago Hospital Chicago, Illinois Steven H. Bowman, MD, FACEP Assistant Professor Department of Emergency Medicine Rush Medical College Program Director Department of Emergency Medicine Cook County (Stroger) Hospital Chicago, Illinois Sean M. Bryant, MD Associate Professor Emergency Medicine Assistant Fellowship Director Toxikon Consortium Cook County (Stroger) Hospital Associate Medical Director Illinois Poison Center Chicago, Illinois Ann Buchanan, MD Assistant Director/Trauma Medical Director Department of Emergency Medicine St. David's Medical Center Austin, Texas Paul E. Casey, MD Instructor in Clinical Medicine Department of Emergency Medicine Rush University Medical Center Chicago, illinois Esther H. Chen, MD Associate Professor Emergency Medicine University of California, San Francisco General Hospital San Francisco, California George Chiampas, DO Assistant Professor, Department of Emergency Medicine Northwestern University Feinberg School of Medicine Team Physician, Northwestern University Medical Director, Bank of America Chicago Marathon Chicago, Illinois Kristine Cieslak, MD Assistant Professor Department of Pediatrics Northwestern University Feinberg School of Medicine Chicago, Illinois Director, Pediatric Emergency Medicine Department of Pediatric Emergency Medicine Children's Memorial at Central DuPage Hospital Winfield, Illinois Michael T. Cudnik, MD, MPH Assistant Professor Department of Emergency Medicine The Ohio State University Medical Center Columbus, Ohio Joanna Wieczorek Davidson, MD Department of Emergency Medicine Cook County (Stroger) Hospital Chicago, Illinois John Davis, MD, PhD Assistant Professor Department of Medicine The Ohio State University Attending Physician Department of Medicine Wexner Medical Center at The Ohio State University Columbus, Ohio Alex de la Fuente, MD Private Practice Everett, Washington Nicole M. Deiorio, MD Associate Professor Department of Emergency Medicine Director, Medical Student Education Oregon Health and Science University Portland, Oregon CONTRIBUTORS E. Paul DeKoning, MD, MS Assistant Professor Emergency Medicine Dartmouth-Hitchcock Medical Center Medical Student Education Director Emergency Medicine Dartmouth Medical School Lebanon, New Hampshire Bradley L. Demeter, MD EMS Physician Emergency Medicine University of Chicago Chicago, Illinois Vinodinee L. Dissanayake, MD Global Toxicology Fellow Emergency Medicine University of Illinois at Chicago and Cook County (Stroger) Hospital Clinical Instructor University of Illinois at Chicago Chicago, Illinois Marc Doucette, MD Associate Professor Emergency Medicine University of Colorado School of Medicine Attending Physician Emergency Medicine St. Anthony Hospital System Denver, Colorado Matthew T. Emery, MD Assistant Professor Emergency Medicine Michigan State University College of Human Medicine Attending Physician Department of Emergency Medicine Spectrum Health Hospital-Butterworth Campus Grand Rapids, Michigan Rakesh S. Engineer, MD Assistant Professor Emergency Medicine Case School of Medicine Attending Physician Emergency Services Institute Cleveland Clinic Cleveland, Ohio Jorge Fernandez, MD Assistant Professor of Clinical Emergency Medicine Department of Emergency Medicine University of Southern California Director of Medical Student Education Department of Emergency Medicine LA County + USC Medical Center Los Angeles, California CONTRIBUTORS Michael T. Fitch, MD, PhD, FACEP, FAAEM Associate Professor Department of Emergency Medicine Wake Forest School of Medicine Winston-Salem, North Carolina Alison R. Foster, MD Department of Emergency Medicine Northwestern University Chicago, illinois Douglas Franzen, MD, M.Ed, FACEP Assistant Professor Department of Emergency Medicine Virginia Commonwealth University Medical Center Richmond, Virginia Casey Glass, MD Assistant Professor Department of Emergency Medicine Wake Forest Health Sciences Winston-Salem, North Carolina David C. Gordon, MD Assistant Professor Division of Emergency Medicine, Department of Surgery Duke University Durham, North Carolina Nihja 0. Gordon, MD Department of Emergency Medicine Cook County (Stroger) Hospital Chicago, illinois Krista A. Grandey, DO Department of Emergencey Medicine Cook County (Stroger) Hospital Chicago, illinois Pilar Guerrero, MD Assistant Professor Department of Emergency Medicine Cook County (Stroger) Hospital Chicago, illinois Marianne Haughey, MD Associate Professor of Emergency Medicine Department of Emergency Medicine Jacobi Medical Center, Albert Einstein College of Medicine Bronx, New York Tarlan Hedayati, MD Assistant Program Director Department of Emergency Medicine Cook County (Stroger) Hospital Assistant Professor Department of Emergency Medicine Rush Medical College Chicago, illinois Corey R. Heitz, MD Assistant Professor Department of Emergency Medicine Virginia Tech Carilion School of Medicine Roanoke, Virginia Ross A. Heller, MD, MBA Associate Professor Department of Surgery/Division of Emergency Medicine Saint Louis University School of Medicine Saint Louis, Missouri Colleen N. Hickey, MD Assistant Professor Department of Emergency Medicine Northwestern University Feinberg School of Medicine Attending Physician Department of Emergency Medicine Northwestern Memorial Hospital Chicago, illinois Katherine M. Hiller, MD, MPH, FACEP Associate Professor Department of Emergency Medicine University of Arizona College of Medicine Tucson, Arizona Russ Horowitz, MD, RDMS Assistant Professor Department of Pediatrics Northwestern University Feinberg School of Medicine Attending Physician, Director Emergency Ultrasound Division of Emergency Medicine Children's Memorial Hospital Chicago, illinois Craig Huston, MD Emergency Physician Department of Emergency Medicine Blessing Hospital Quincy, illinois Harry C. Karydes, DO Assistant Professor Attending Physician Department of Emergency Medicine Rush Medical College Chicago, illinois Elizabeth W. Kelly, MD Assistant Professor Department of Emergency Medicine Wake Forest School of Medicine Winston-Salem, North Carolina Chad S. Kessler, MD Section Chief, Emergency Medicine Department of Medicine Jesse Brown VA Medical Center Chicago, illinois Sorabh Khandelwal, MD Associate Professor Department of Emergency Medicine Assistant Dean for Clinical Sciences College of Medicine The Ohio State University Columbus, Ohio Basem F. Khishfe, MD Department of Emergency Medicine Cook County (Stroger) Hospital Chicago, Illinois Brian C. Kitamura, MD Emergency Medicine Maricopa Integrated Health System Phoenix, Arizona Nicholas E. Kman, MD, FACEP Assistant Professor Department of Emergency Medicine The Ohio State University College of Medicine Columbus, Ohio Amy V. Kontrick, MD Assistant Professor Emergency Medicine Northwestern University Feinberg School of Medicine Chicago, Illinois Carl M. Kraemer, MD, FAAEM, FACEP Assistant Professor Department of Emergency Medicine Saint Louis University School of Medicine St. Louis, Missouri Brian Krieger, MD Department of Emergency Medicine Cook County (Stroger) Hospital Chicago, Illinois Rashid E. Kysia, MD, MPH Attending Physician Department of Emergency Medicine Cook County (Stroger) Hospital Assistant Professor Rush Medical College Chicago, Illinois Patrick M. Lank, MD Fellow Division of Medical Toxicology Toxikon Consortium Cook County (Stroger) Hospital Attending Physician Department of Emergency Medicine Northwestern Memorial Hospital Chicago, Illinois CONTRIBUTORS William B. Lauth, MD, FACEP Clinical Professor Department of Emergency Medicine Rosalind Franklin University, The Chicago Medical School Attending Physician Department of Emergency Medicine Captain James A. Lovell Federal Health Care Center North Chicago, Illinois Moses S. Lee, MD, FAAEM, FACEP Assistant Professor Emergency Medicine Rush Medical College Attending Physician Emergency Medicine Cook County (Stroger) Hospital Chicago, Illinois Trevor J. Lewis, MD Associate Professor Department of Emergency Medicine Rush Medical College Medical Director Emergency Department Cook County (Stroger) Hospital Chicago, Illinois Nathan Lewis, MD Assistant Professor Department of Emergency Medicine Virginia Commonwealth University School of Medicine Richmond, Virginia Chuang-yuan Lin, MD Department of Emergency Medicine Cook County (Stroger) Hospital Chicago, Illinois Jenny J. Lu, MD, MS Assistant Professor Department of Emergency Medicine, Division of Medical Toxicology Cook County (Stroger) Hospital Chicago, Illinois David E. Manthey, MD Professor Emergency Medicine Wake Forest School of Medicine Winston-Salem, North Carolina Anitha E. Mathew, MD Clinical Instructor Department of Emergency Medicine Emory University School of Medicine Attending Physician Department of Emergency Medicine Emory University Hospital and Grady Memorial Hospital Atlanta, Georgia xii Alisa A. McQueen, MD Assistant Professor CONTRIBUTORS Department of Pediatrics, Section of Pediatric Emergency Medicine University of Chicago Pritzker School of Medicine Attending Physician Pediatric Emergency Medicine University of Chicago Comer's Children Hospital Chicago, illinois Biswadev Mitra, MBBS, MHSM, PhD, FACEM Emergency & Trauma Centre The Alfred Hospital Melbourne, Australia Brooks L. Moore, MD Assistant Professor Department of Emergency Medicine Emory University School of Medicine Attending Physician Department of Emergency Medicine Emory University Hospital and Grady Memorial Hospital Atlanta, Georgia Tom Morrissey, MD, PhD Associate Professor Department Emergency Medicine University of Florida-Jacksonville Jacksonville, Florida Jordan B. Moskoff, MD Associate Medical Director Department of Emergency Medicine Cook County (Stroger) Hospital Assistant Professor Department of Emergency Medicine Rush Medical College Chicago, illinois Mark B. Mycyk, MD, FACEP, FACMT Associate Professor Department of Emergency Medicine Rush Medical College Northwestern University Feinberg School of Medicine Attending Physician Department of Emergency Medicine Cook County (Stroger) Hospital Chicago, illinois Isam F. Nasr, MD, FACEP Assistant Professor Department of Emergency Medicine Rush Medical College Attending Physician Department of Emergency Medicine Cook County (Stroger) Hospital Chicago, illinois Michael E. Nelson, MD, MS Attending Physician, Medical Toxicology Fellow Department of Emergency Medicine Cook County (Stroger) Hospital Chicago, illinois Attending Physician Department of Emergency Medicine Northshore University Health System Evanston, Illinois Erik K. Nordquist, MD Assistant Professor Department of Emergency Medicine Cook County (Stroger) Hospital Chicago, illinois Paula E. O ldeg, MD, FACEP Attending Physician Department of Emergency Medicine West Suburban Medical Center Oak Park, Illinois Adjunct Clinical Instructor Department of Emergency Medicine Rush Medical College Chicago, illinois S. Margaret Paik, MD Assistant Professor of Pediatrics Department of Pediatrics Associate Section Chief, Pediatric Emergency Medicine The University of Chicago Comer Children's Hospital Chicago, illinois Lisa R. Palivos, MD Assistant Professor Department of Emergency Medicine Rush Medical College Attending Physician Department of Emergency Medicine Cook County (Stroger) Hospital Chicago, illinois Jonathon D. Palmer, MD Assistant Professor Department of Emergency Medicine University of Arkansas for Medical Sciences Little Rock, Arkansas Matthew S. Patton, MD Department of Emergency Medicine Northwestern University Feinberg School of Medicine Chicago, illinois Rahul G. Patwari, MD Medical Student Clerkship Director Assistant Professor Attending Physician Department of Emergency Medicine Rush Medical College Chicago, illinois Monika Pitzele, MD, PhD Attending Physician Department of Emergency Medicine Mount Sinai Hospital Chicago, Illinois Henry Z. Pitzele, MD, FACEP Deputy Director Emergency Medicine Jesse Brown VA Medical Center Clinical Assistant Professor Department of Emergency Medicine University of Illinois at Chicago Chicago, Illinois Natalie Radford, MD Associate Professor Department of Clinical Medicine Florida State University Attending Physician Bixler Emergency Department Tallahassee Memorial Hospital Tallahassee, Florida Christopher Reverte, MD Chief Resident Department of Emergency Medicine LA County + USC Medical Center Los Angeles, California Attending Physician Department of Emergency Medicine St. Luke's-Roosevelt New York, New York Neil Rifenbark, MD Department of Emergency Medicine University of Southern California Department of Emergency Medicine LA County + USC Medical Center Los Angeles, California Rebecca R. Roberts, MD Director, Research Division Attending Physician Department of Emergency Medicine Cook County (Stroger) Hospital Chicago, Illinois Sarah E. Ronan-Bentle, MD, MS, FACEP Assistant Professor Department of Emergency Medicine University of Cincinnati College of Medicine Attending Physician Center for Emergency Care University Hospital Cincinnati, Ohio CONTRIBUTORS David H. Rosenbaum, MD, FAAEM Attending Physician Department of Emergency Medicine WakeMed Health and Hospitals Raleigh, North Carolina Adjunct Professor Department of Emergency Medicine xiii University of North Carolina School of Medicine Chapel Hill, North Carolina Christopher Ross, MD, FRCPC, FACEP, FAAEM Assistant Professor Department of Emergency Medicine Associate Chair Planning, Education, and Research Cook County (Stroger) Hospital Chicago, Illinois John Sarko, MD Attending Physician Department of Emergency Medicine Maricopa Medical Center Assistant Professor Department of Emergency Medicine University of Arizona Phoenix School of Medicine Phoenix, Arizona Shari Schabowski, MD Assistant Professor Department of Emergency Medicine Rush Medical College Attending Physician Department of Emergency Medicine Cook County (Stroger) Hospital Chicago, Illinois Conor D. Schaye, MD, MPH Department of Emergency Medicine Northwestern Memorial Hospital Chicago, Illinois Michael A. Schindlbeck, MD, FACEP Assistant Professor Department of Emergency Medicine Rush Medical College Assistant Residency Director Cook County (Stroger) Hospital Chicago, Illinois Suzanne M. Schmidt, MD, FAAP Clinical Instructor Department of Pediatrics Northwestern University Feinberg School of Medicine Attending Physician Department of Pediatrics Ann & Robert H. Lurie Children's Hospital of Chicago Chicago, Illinois xiv T heresa M. Schwab, MD Attending Physician Department of Emergency Medicine Advocate Christ Medical Center Oak Lawn, Illinois Assistant Professor Department of Emergency Medicine University of Illinois at Chicago Chicago, Illinois Brian R. Sellers, MD Department of Emergency Medicine CONTRIBUTORS Northwestern University Feinberg School of Medicine Chicago, Illinois Emily L. Senecal, MD Clinical Instructor Department of Emergency Medicine Harvard Medical School Attending Physician Department of Emergency Medicine Massachusetts General Hospital Boston, Massachusetts Michelle Sergei, MD Assistant Professor Department of Emergency Medicine Rush Medical College Attending Physician Department of Emergency Medicine Cook County (Stroger) Hospital Chicago, Illinois Scott C. Sherman, MD Medical Student Clerkship Director PA Residency Director Associate Residency Director Department of Emergency Medicine Cook County (Stroger) Hospital Rush Medical College Chicago, Illinois Jeffrey N. Siegelman, MD Assistant Professor Department of Emergency Medicine Emory University Atlanta, Georgia Jessica Sime, MD Department of Emergency Medicine Union Memorial Hospital Baltimore, Maryland Lauren M. Smith, MD Assistant Professor Department of Emergency Medicine Rush Medical College Attending Physician Department of Emergency Medicine Cook County (Stroger) Hospital Chicago, Illinois William T homas Smith, MD Emergency Medicine Oregon Health and Sciences University Portland, Oregon Shannon E. Staley, MD Pediatric Emergency Medicine Fellow Department of Pediatrics University of Chicago Comer Children's Hospital Chicago, Illinois Christine R. Stehman, MD Clinical Fellow Department of Surgery, Division of Trauma, Burn, and Surgical Critical Care Associate Physician Department of Emergency Medicine Brigham and Women's Hospital, Harvard Medical School Boston, Massachusetts Harsh Sule, MD, FAAEM, FACEP Assistant Professor Department of Emergency Medicine Thomas Jefferson University & Hospitals Philadelphia, Pennsylvania Gim A. Tan, MBBS, FACEM Adjunct Lecturer Department of Emergency Medicine Monash University Senior Emergency Physician Emergency and Trauma Centre The Alfred Hospital Melbourne, Australia Katie L. Tataris, MD Department of Emergency Medicine Cook County (Stroger) Hospital Chicago, Illinois Matthew C. Tews, DO Associate Professor Department of Emergency Medicine Medical College of Wisconsin Milwaukee, Wisconsin CONTRIBUTORS S. Spencer Topp, MD Assistant Professor Department of Emergency Medicine University of Florida Health Science Center-Jacksonville Jacksonville, Florida Brandon C. Tudor, MD Private Practice Everett, Washington Katrina R. Wade, MD, FAAEM, FAAP Associate Professor Department of Surgery, Emergency Medicine Division Assistant Professor, Pediatrics Department of Pediatrics Saint Louis University School of Medicine St. Louis, Missouri David A. Wald, DO Professor of Emergency Medicine Medical Director, William Maul Measey Institute for Clinical Simulation and Patient Safety Temple University School of Medicine Philadelphia, Pennsylvania Joseph Walline, MD Assistant Professor Department of Surgery, Division of Emergency Medicine Saint Louis University School of Medicine Saint Louis, Missouri Joseph M. Weber, MD EMS Medical Director Department of Emergency Medicine Cook County (Stroger) Hospital Assistant Professor of Emergency Medicine Rush Medical College Chicago, Illinois Joanne C. Witsil, PharmD, RN, BCPS Adjunct Clinical Assistant Professor Department of Pharmacy Practice University of Illinois at Chicago Clinical Pharmacist Department of Emergency Medicine Cook County (Stroger) Hospital Chicago, Illinois Kathleen A. Wittels, MD Instructor in Medicine Department of Emergency Medicine Harvard Medical School Associate Clerkship Director Department of Emergency Medicine Brigham and Women's Hospital, Harvard Medical School Boston, Massachusetts Lynne M. Yancey, MD, FACEP Associate Professor Department of Emergency Medicine University of Colorado School of Medicine Denver, Colorado Attending Physician Department of Emergency Medicine University of Colorado Hospital Aurora, Colorado Leslie S. Zun, MD Professor Emergency Medicine Chicago Medical School Chair Emergency Medicine Mount Sinai Hospital Chicago, Illinois Preface We wrote this book because we remember our own experiences as medical students and junior residents working in the emergency department (ED). The ED is a unique environment that requires knowledge and skills often not covered in medical or physician assistant school. In this book, we attempt to create a resource for the medical student, physician assistant, nurse practitioner, and junior-level resident to use to get a grasp on the issues and scope of problems that they will confront while working in the ED. The book's length and format are designed to allow the student and practitioner to begin to digest the broad range of topics inherent to emergency medicine (EM). Each chapter begins with a section on Key Points, followed by an Introduction, Clinical Presentation (History and Physical Examination), Diagnostic Studies, Medical Decision Making, Treatment, and Disposition. Whenever possible, we tried to give practical information regarding drug dosing, medical decision-making thought processes, treatment plans, and dispositions that will actually allow you to function more comfortably in the clinical environment. The diagnostic algorithms are a unique feature that attempt to simplify the problem and point the clinician in the right direction. The book has 19 sections and 98 chapters that cover the entire contents of the EM clerkship curriculum (Acad Emerg Med. 201 0; 1 7:638-643). The authors are all practicing emergency physicians and EM educators from throughout the country. For medical student clerkship directors, we believe that this text is the perfect book for the student to pick up and digest during a 4-week rotation. In summary, we hope this book will enhance the emergency medicine experience of all its users. xvi Scott C. Sherman, MD Joseph M. Weber, MD Michael Schindlbeck, MD Rahul Patwari, MD Download pdf

Download Handbook of Nonprescription Drugs.pdf

Handbook of Nonprescription Drugs.pdf - 33.4 MB

 

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Self­Care Self­care is the independent act of preventing, diagnosing, and treating one’s illnesses without seeking professional advice. Preventive self­care involves maintaining well­being and appearance through exercise and a healthy lifestyle. For many individuals, a healthy lifestyle includes controlling their diet; taking vitamins, minerals, and herbal supplements; participating in regular exercise and keeping fit; and maintaining their appearance by using dental, skin, and hair care products. However, sickness self­care for individuals involves diagnosing their conditions and obtaining products for the goal of mitigating illness and relieving symptoms. Examples of sickness self­care include use of dietary options (e.g., warm soup for a cold); use of devices for both disease assessment (e.g., home blood glucose meters and pregnancy tests) and treatment (e.g., ice packs, first­aid bandages, vaporizers, and nasal strips); and use of nonprescription medications. The use of sickness self­care products is limited to mild illness or short­term management of illness, and most products warn users to contact a health care provider if conditions do not improve within a short period of time. For the provision of sickness self­care, one individual from each household usually plays a leading role in adopting a course of action. This individual must determine whether a health care provider should be consulted or whether the use of home remedies and self­care will suffice. Furthermore, the number of individuals involved in choosing the most appropriate self­care option is increasing because of the growth of the U.S. geriatric population, whose members are known as high users of nonprescription medications. 1 Individuals responsible for providing self­care for themselves or family members rely on knowledge and experience to guide their decisions. For better or worse, there is no shortage of information, given the wealth of health­related self­help books, newspaper feature articles, magazine and television advertisements, magazine articles, radio programs, instructional tapes and CDs, DVDs, and Internet sites—all of which provide self­care advice. The availability of coupons indirectly provides information and encourages cost savings for trying a product. The abundance of available health­related information, especially from the Internet, helps individuals become more “selfempowered” to address their health care issues and leads to aggressive marketing and use of self­care alternatives. The quality of the information ranges from excellent to very poor. Although it is more accepted today for individuals to attempt to manage their health­related issues rather than to consult a health care provider, the concern is whether they are making appropriate and informed decisions. Furthermore, all this health information can become overwhelming, driving some individuals to seek advice from family and friends. This well­intentioned advice can be problematic because it is often biased, and most individuals are not sufficiently informed or qualified to consider another’s health conditions or medications before making a recommendation. They simply state what has worked best for them and fail to consider how their approach might apply to someone else. Commercial products used for preventive or sickness self­care are often classified together as health and beauty care (HBC) products. Staggering numbers of HBC products are available. Although access to quality HBC products is crucial to the goal of self­care, the vast number of similar, competing products makes appropriate selection difficult. Yet, in one consumer poll in which 66% of adults believed that the wide range of competing products made selection difficult, less than half (43%) said they consulted a pharmacist before making a purchase. 2 The pharmacist plays a crucial role in assisting patients who are seeking both preventive and sickness self­care products. The practicing pharmacist has the expertise to screen patient health information and apply his or her knowledge and training to select products according to individual health care needs. Therefore, for pharmacies to provide pharmacist­assisted self­care, only quality HBC products should be stocked, and a pharmacist should be readily available for patients seeking assistance. More and more pharmacies are changing their floor layout and design and staffing to ensure that a pharmacist is available and easily accessible to patients seeking advice in the nonprescription drug aisles. At times, this change coincides with the implementation of a retail clinic staffed by a nurse practitioner or a physician assistant as a primary care provider. Self­Medication Self­medication is often the most sought­after first level of self­care. As self­care has increased, so has the practice of self­medication with vitamins (i.e., nutritional dietary supplements), natural products (i.e., herbal/botanical and nonherbal dietary supplements [e.g., glucosamine]), and nonprescription medications. Factors that help drive reliance on self­medication include (1) growth of the aging population, (2) decreased availability of primary care providers, (3) increased costs of health care, and (4) high proportion of underinsured or uninsured people in the United States. Easy accessibility, convenience, and cost­effectiveness of self­medication products ensure their essential role in the U.S. health care system.

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Results of a 2002 survey conducted for the National Council on Patient Information and Education (NCPIE) 3 illustrate how ubiquitous the use of nonprescription medications has become. According to the survey, 59% of Americans had taken at least one nonprescription medication in the preceding 6 months. Table 1­1 illustrates some of the conditions that are self­treatable with nonprescription medications. Conditions and symptoms commonly treated with nonprescription medications include the following: Pain (78%) Cough/cold/flu/sore throat (52%) Allergy/sinus problems (45%) Heartburn/indigestion (37%) Constipation/diarrhea/gas (21%) Minor infections (12%) Skin problems (10%) Approximately 20% of Americans believe that they are consuming more nonprescription medications and taking them more frequently than they did 5 years earlier. 3 This increase in nonprescription drug use may reflect a consumer belief that self­medication is safe. Additional data collected in the survey “Your Health at Hand: Perceptions of Over­the­Counter Medicine in the U.S.” supports the view that consumers and physicians are confident in their use of nonprescription medications. 4 These survey results show the following: 93% of physicians believe it is important that medications for minor ailments be available over the counter. 96% of consumers believe nonprescription medications make it easy to care for minor medical ailments. 87% of physicians and 89% of consumers believe nonprescription medications are an important part of overall and family health care. 93% of adults prefer to treat minor ailments with nonprescription medications before seeking professional care. 88% of physicians recommend patients address minor ailments with self­care, including nonprescription medications before seeking professional care. TABLE 1­1 Selected Medical Disorders Amenable to Nonprescription Drug Therapy a Abrasions Aches and pains (general, mildmoderate) Acidity, stomach Acne Allergic reactions (mild) Allergic rhinitis Anemia (after diagnosis by a health care provider) Arthralgia Asthma (after diagnosis by a health care provider) Athlete’s foot Bacterial infection (dermatologic, mild) Blisters Blood pressure monitoring Boils Bowel preparation (diagnostic) Burns (minor, thermal) Calluses Candidal vaginitis Canker sores Carbuncles Chapped skin Cold sores Colds (viral upper respiratory infection) Congestion (chest, nasal) Constipation Contact lens care Contraception Corns Cough Cuts (superficial) Dandruff Decongestant, nasal Dental care Dermatitis (contact) Diabetes mellitus (insulin, monitoring equipment, supplies) Diaper rash Diarrhea Dry skin Dyslipidemia Dysmenorrhea Dyspepsia Fever Flatulence Gastritis Gingivitis Hair loss Halitosis Hangover relief, morning Head lice Headache Heartburn Hemorrhoids Herpes Impetigo Indigestion Ingrown toenails Insect bites and stings Insomnia Jet lag Jock itch Migraine Motion sickness Myalgia Nausea Nutrition (infant) Obesity Occult blood, fecal (detection) Ostomy care Ovulation prediction Periodontal disease Pharyngitis Pinworm infestation Premenstrual syndrome Prickly heat Psoriasis Ringworm Seborrhea Sinusitis Smoking cessation Sprains Strains Stye (hordeolum) Sunburn Teething Thrush Toothache Vomiting Warts (common and plantar) Xerostomia Wound careDownload pdf

The pertinent nonprescription medication(s) for a particular disorder may serve as primary or major adjunctive therapy. Source: U.S. Food and Drug Administration. Status of OTC rulemaking. February 17, 2011. Accessed at http://www.fda.gov/Drugs/DevelopmentApprovalProcess/DevelopmentResources/Over­theCounterOTCDrugs/StatusofOTCRulemakings/default.htm, June 2014. A major 2012 survey of more than 3200 individuals provides information regarding the value of nonprescription medications and their contribution to cost savings and quality care in our health care system. 5 These data showed the following: Availability of nonprescription products provides $102 billion in annual savings relative to alternatives and increased access to medications. Each dollar spent on nonprescription medications saves $6­$7 for the health care system. In the previous 12 months, 79% of consumers (or approximately 240 million people) took a nonprescription drug in the categories of allergy, analgesics, antifungals, cough/cold/flu, lower gastrointestinal, upper gastrointestinal, and medicated skin. 180 million individuals would seek treatment from a primary care provider if nonprescription medications were not available. Self­medication plays an increasing role as adjunctive therapy for chronic diseases that are managed with prescription medications. Examples include low­dose aspirin for reducing heart attack risk, fish oil (omega­3 fatty acids) to help manage certain dyslipidemias, and glucosamine with chondroitin to help relieve symptoms of osteoarthritis. However, the use of nonprescription products as adjunctive therapy comes with potential risks associated with incorrect product selection. For example, many patients who require daily low­dose aspirin do not fully understand the differences between the many aspirin products. There are various strengths (low dose, regular, and extra strength) and products (chewable, buffered, and enteric coated). Selection of the wrong product could result in adverse reactions (e.g., gastritis or ulcer) or drug­drug interactions (e.g., warfarin and blood pressure medications). The pharmacist plays an important role in helping patients select the correct products for their condition. Options for Self­Medication Three general categories of products are available for self­medication: (1) nonprescription medications, (2) nutritional dietary supplements, and (3) natural products and homeopathic remedies. Nonprescription Medications Nonprescription medications are regulated by the Center for Drug Evaluation and Research, a division of the U.S. Food and Drug Administration (FDA)—the same agency that regulates prescription drug products. As such, nonprescription medications are held to the same drug product formulation (e.g., purity and stability), labeling, and safety (benefits outweigh risks) standards as those for prescription medications. A complete discussion of the monograph system can be found in Chapter 4. It is worth noting that, although nonprescription medications are regulated in a manner equivalent to that of prescription medications, the sales of nonprescription medications are not limited to pharmacies. They are also available in retail establishments such as discount stores, supermarkets, and gas station quick stops and on a plethora of Internet sites. The provisions of the 1951 Durham­Humphrey Amendment to the Food, Drug, and Cosmetic Act of 1938 gives FDA the final authority to categorize a medication as prescription or nonprescription. FDA deems nonprescription medications safe and effective when they are used without a prescriber’s directive and oversight. In addition, these products have the following characteristics: they have a low potential for misuse and abuse; patients can use them for self­diagnosed conditions; they are adequately labeled; and they do not require access to a health care provider for safe and effective use. 6 The estimated number of available FDA­approved nonprescription drug products is 100,000, including more than 1000 active ingredients that cover more than 80 therapeutic categories. Your Health at Hand Book: Guide to OTC Active Ingredients in the United States provides an extensive list of active ingredients, therapeutic categories, and examples of brand name products. 7 Sales of nonprescription medications in 2011 were estimated at $17.4 billion dollars. 8 Sales of the top 15 therapeutic categories of nonprescription medications for 2011 are shown in Figure 1­1. 9 Not surprisingly, the dollars spent, as shown in this figure, correspond to what consumer surveys have reported as the most common conditions managed with self­care. For example, in a survey in which consumers were asked what health problems they had experienced in the preceding 6 months, the most frequent responses were muscle/back/joint pain and cough/cold/flu/sore throat (both categories at 48%), with headache and 6/1/2015 PharmacyLibrary | Print: Chapter 1. Self­Care and Nonprescription Pharmacotherapy http://www.pharmacylibrary.com.ezproxy.roosevelt.edu:2048/popup.aspx?aID=785002&print=yes_chapter 4/16 heartburn/indigestion trailing at 43% and 32%, respectively. 10 The correlation between common types of illnesses and dollars spent implies that many Americans provide self­care for those conditions using nonprescription medications. Nutritional Dietary Supplements The Dietary Supplement Health and Education Act of 1994 amended the 1983 Food, Drug, and Cosmetic Act to establish standards with respect to dietary supplements. The amendment defined dietary supplements as products that are intended to supplement the diet and that bear or contain one or more of the following dietary ingredients: (1) a vitamin, (2) a mineral, (3) an herb, or (4) an amino acid. Results from a recent National Health and Nutrition Survey indicate that more than 50% of adults used one or more dietary supplements between 2003 and 2006. 11 A survey of college students found that almost three­quarters (71%) had used an herbal or nutritional supplement and that 61% had used both a nonprescription product and a nutritional or herbal supplement within the past year. 12 (See Chapters 50, 51, and 52 for further information on dietary supplements.) Natural Products and Homeopathic Remedies Because of factors such as high health care costs and restricted access to conventional health care providers, many patients seek care from providers of complementary and alternative medicine (CAM). The National Center for Complementary and Alternative Medicine and the National Center for Health Statistics reported survey results on Americans’ use of CAM. 13 Approximately 38% of adults and 12% of children reported receiving some form of CAM therapy in 2007. Some of the most common forms of CAM therapy were natural products (18%), deep breathing (13%), meditation (9%), chiropractic and osteopathic care (9%), massage (8%), and yoga (6%). Adults spent approximately $34 billion out of pocket to visit CAM providers and purchase products. 14 Self­care is a component of many CAM therapies. By definition, the term dietary supplement includes both herbal and natural products. In 2011, the total estimated sales of herbal products were $5.03 billion, with the top­selling supplements being cranberry, soy, saw palmetto, garlic, and echinacea. 15 A 2002 National Health Survey indicated that about 13% of elderly patients had used an herbal supplement during the preceding year. 16 The use of combined herbal and conventional therapy raises safety concerns because 51% of patients failed to inform their health care provider about their herbal therapy. 11 These safety concerns include the potential for herbal supplement­drug interactions. Lexi­Comp’s Lexi­Interact database (Hudson, Ohio, Lexi­Comp, Inc., 2013) lists several hundred herbdrug interactions, with garlic, St. John’s wort, the various ginseng products, and Ginkgo biloba leading the way. Therefore, individuals who take prescription or nonprescription medications should consult with a pharmacist or other health care provider before self­medicating with herbal supplements.

12/27/23

 


A day book is ideal as it has the necessary ruled lines. In

your record put the date, reference number, patient’s

name, name of the referring doctor, investigations asked

for, reports given and payment status (if privately owned

laboratory).

Laboratory Reporter

An ideal laboratory computer program helps in reporting

and recording diagnostic center, pathology lab and other

diagnostic imaging fields. The program should also keep

history records of the patients. It must have facilities of

making and reporting profiles, e.g. lipid, renal, cardiac,

hepatic and diabetic profile. A program can be called ideal if:

FIG. 1.29: Serological water bath

(Courtesy: Yorco Sales Pvt. Ltd)

FIG. 1.30A: Incubator

(Courtesy: Yorco Sales Pvt. Ltd)

FIG. 1.30B: Hot air oven

(Courtesy: Yorco Sales Pvt. Ltd)

It Reduces Overload

¾ Avoids manual operations by printing booking slips,

receipts, bills, envelopes, etc.

¾ Prints daily register of patients

¾ Prints rate lists

¾ Reports only the tests required and not the whole group

of tests

¾ A comprehensive reporting option for day end

operations including daily collection report and doctor

wise daily collection.

28 Concise Book of Medical Laboratory Technology: Methods and Interpretations Graphs

¾ Prints graphs, e.g. GTT for to the point reporting

¾ Can make/design your own graphs

¾ Can see the graph on screen as well as print.

Accounts

¾ Maintains your bank, cash accounts

¾ Provides all ledgers

¾ Makes trial balance for final accounts.

Address Manager

¾ A mail list program, keeps address details.

¾ Provides easy working on the basis of name

¾ Keeps addresses for Labmate program of referencing

doctors, patients and reporting doctors

¾ Prints address directory with telephone numbers, etc.

¾ Prints labels for sticking on your mail

¾ Can group your addresses as per nature of address such

as friend, relative, doctor, patient, etc.

The features given above are complete. All records

can be retrieved date wise or name wise. Any program

that provides the above-mentioned capabilities can be

considered as an ideal laboratory reporter.

Caution: All medical electronic diagnostic devices need a

stable constant voltage, therefore, proper protective cover

must be provided. CVT (constant voltage transformer),

servo stabilizers, and UPS (uninterrupted power supply)

should be installed in the mainline or with specific

instruments.

¾ It helps referencing doctor

¾ Provides clear reports with normal values

¾ Abnormal values are underlined or highlighted

automatically

¾ Prints history reports of the patients.

It Makes Working Easy

¾ Reports as per your own method of grouping of tests,

profiles, etc.

¾ Automatic calculation of charges

¾ Keeps list of referencing doctors

¾ Maintains daily collection on referencing doctor/

institution

¾ Provides workload report.

The Computerized System is Easy to Operate

¾ Simple menu-based operations and does not require

any detailed knowledge of computers

¾ Help facility at every stage of working for beginners

¾ Can find a patient detail based on of reference number,

name, date and referencing doctor.

Features and Provisions

¾ Keeps the results for as long as you want

¾ Keeps normal values for male/female and adult/child

for all tests

¾ Can change any normal value as per your equipment,

techniques and methods

¾ Provision for reporting by different doctors

¾ Reports can be printed on simple paper or on preprinted

letter heads (computer stationery).

2

 


Sterilization

C H A P T E R

The terms sterilization and disinfection are used to indicate

the treatment of material so as to destroy or otherwise

eliminate any living organisms present. However, the

term sterilization is used where physical methods are

used and disinfection is used where chemical agents are

made use of.

METHODS COMMONLY USED FOR STERILIZATION

The methods used commonly in practice are:

1. Killing organisms by heat: Heat may be dry or moist

2. Destroying organisms by employing chemical antiseptics,

e.g. lysol, phenol, perchloride of mercury, etc.

3. Removing organisms mechanically by filtration, e.g.

Seitz, unglazed porcelain.

Sterilization by Heat

Adequate heat is the most certain and rapid method for

sterilization. The time needed for sterilization is inversely

related to the temperature of exposure—the higher the

temperature, the shorter the time needed. High temperature

kills bacteria by coagulating their proteins. Different

types of bacteria show considerable differences in heat

susceptibility. In general, vegetative forms are destroyed

at lower temperatures, whereas high temperatures are

needed for sporing organisms.

Dry Heat

This is the preferred method for sterilizing glassware, e.g.

of glass syringes and of materials such as oils, jellies and

powders which are impervious to steam. Dry heat requires

a much higher temperature or a much longer time at the

same temperature than does moist heat. Dry heat can be

used in the following ways:

Flaming

The articles are passed through the Bunsen flame, without

letting them become red hot. It is used for scalpels, needles,

mouths of culture tubes, glass slides, coverslips and points

of forceps. Only the surfaces actually touched by the flame

are sterilized.

Red Heat

Platinum loops, inoculating wires and needles are heated

in the Bunsen flame until red hot.

Hot Air Oven

These are electrically heated and thermostatically

controlled. The oven itself is a double-walled steel chamber

with a stout door. The top or side contains a ventilator

which is left open during sterilization to disperse any

moisture or volatile matter. Air circulates within the oven

by convection currents. Suitable sterilizing times in the

hot air oven are 3 hours at 140°C, 1 hour at 160°C and

30 minutes at 180°C. All dry glassware, such as test tubes,

petri-dishes, flasks, pipettes and throat swabs, etc. are

made sterile by using hot air oven.

This method is not suitable for sterilizing culture

media, liquids, rubber connections, glass to metal fitting

and fabrics, e.g. masks, towels or gowns.

Moist Heat

Temperature

A temperature of 60 to 65°C kills most vegetative bacteria

(made use of in pasteurization of milk and preparation of

vaccines).

Boiling

Boiling is frequently used for sterilizing syringes, etc. but is

not adequate as many spores withstand this temperature.

30 Concise Book of Medical Laboratory Technology: Methods and Interpretations

Steam

Steam is the most effective technique of moist heat

sterilization. Steam may be employed in three ways.

Steam at 100°C

The apparatus used commonly is called Koch’s steamer. It

has a vertical metal cylinder with a conical lid. It is fitted

with a thermometer and has a small opening for escape

of steam.

Sterilization by free steam can be done in two ways.

Prolonged exposure: For 1½ hours, used for broth or

nutrient agar.

Intermittent heat or tyndallization: It involves exposure for

20 minutes on three successive days and is used to sterilize

sugars and gelatin which decompose on higher temperatures.

Principle: Spores would germinate after first steaming and

destroyed on the next, three steamings would eliminate all

spores and their vegetative forms.

Low Temperature Steam

This method is employed for sterilizing materials (blankets, polyethene tubing, etc.), which would be damaged at

higher temperatures.

Steam at Temperatures above 100°C (Autoclaving)

Autoclaves are made of strong metal jackets; strong enough

to withstand high pressures required (Figs 2.1A to C). The

autoclave door is hermetically sealed. It has a safety valve

set to blow off at a predetermined pressure. The principle

is that water boils when its vapor pressure is equal to the

 


a. Specimen bottles—with top screws, e.g. the

universal type containers.

b. Reagent bottles—have ground glass or plastic

stoppers, available in different sizes and may be

made of amber colored glass (Figs 1.21A and B).

c. Drop bottles—fitted with special tops through

which drops can be delivered (Fig. 1.22).

4. Funnels—used to hold filter papers when filtering fluids

or for pouring liquids into narrow neck containers

(Figs 1.23A and B).

5. Cylinders—used for measuring liquids, they have a

pouring spot (Fig. 1.24).

6. Tubes—are of various sizes; of the test tube or

centrifuge (conical) type, with or without a top rim

(Figs 1.25 and 1.26).

7. Pipettes—are used to measure and deliver a given

volume of fluid.

FIG. 1.17: Conical flasks

FIG. 1.18: Volumetric flasks

FIGS 1.19A AND B: (A) Round bottomed flask and

(B) Flat bottomed flask

FIG. 1.20: Beakers

A B

Laboratory 25

FIG. 1.21A AND B: (A) Specimen bottles and (B) Reagent bottles

A B

FIG. 1.22: Drop bottles

a. Volumetric pipettes—have a bulb shape in the

stem. Each pipette is marked to show the given

volume of fluid, it contains or delivers (Figs 1.27A

and B).

b. Graduated pipettes—are of various sizes. They

may be of the non-blow out or the blow out

type.

c. Blood pipettes—have a white back and include

the 0.02 mL pipette used for hemoglobin, red cell

and platelet counts, and also the 0.05 mL pipette

for white cell counts (Fig. 1.28A).

 FIGS 1.23A AND B: (A) Separating funnel and (B) Funnel

A B

FIG. 1.24: Measuring cylinder

d. Pasteur pipettes—have multiple uses. They are

not graduated or marked. These can be bought

or made in the laboratory (Fig. 1.28B).

Other Necessary Equipments

Serological Water Bath

It is electrically heated and has a thermostatic temperature

regulator. It can provide temperature ranging from room

temperature to 100°C. Various sizes to suit various

workloads are available (Fig. 1.29).

26 Concise Book of Medical Laboratory Technology: Methods and Interpretations FIG. 1.25: Test tubes

FIG. 1.26: Centrifuge tubes

FIGS 1.27A AND B: (A) Volumetric pipette and (B) Measuring pipette

A B

FIGS 1.28 A AND B: (A) Blood pipettes and (B) Pasteur pipttes

A B

Incubator

Works on electricity and regulates temperature thermostatically. Necessary for various investigations where body

temperature 37°C (or otherwise) incubation is required

(Fig. 1.30A).

Hot Air Oven

This is used for drying and sterilizing glassware. This too

is thermostatically controlled and electrically heated. It

looks like an incubator (Fig. 1.30B).

Reporting Laboratory Tests and Keeping Records

Standardization

Standardization in the reporting of laboratory tests

contributes to the efficiency of the laboratory service

and is of great value when patients are referred from one

place to another. Whenever possible, request forms and

other laboratory printed stationery should be prepared

and issued by a central stationery office.

Laboratory 27

Use of Rubber Stamps

When stationery is not supplied from a central source,

standardization in presenting and reporting results can be

achieved by the use of rubber stamps. Adequate ink must

be used and the stamp must be positioned carefully.

Format

The top part of the report card must prominently give the

name, address and telephone numbers of the laboratory. It

should then have place for printing the patient’s name, age,

sex, name of the referring doctor, the laboratory reference

number and date. Next, the title of the report should be

mentioned, e.g. urinalysis, stool examination, hematology,

biochemistry, etc. After this, print the investigation name,

leave space for patient’s values, print normal values

followed by the units. The report must end with the

signatures of the person in-charge of the laboratory.

Keeping Records in the Laboratory

A record of all test results must be kept by the laboratory

as carbon copies, work sheets, or in simple exercise books.

 


Importance

1. For identifying mycobacteria.

2. It is used extensively in fluorescent antibody techniques

used in parasitology and bacteriology. FIG. 1.11: The principle of dark ground illumination

22 Concise Book of Medical Laboratory Technology: Methods and Interpretations 3. It is also used widely in histopathology of kidney, skin,

etc. where immune/autoimmune basis of disease is

expected. In fact, anything can be confirmed with

high degree of sensitivity and specificity, if antibodies

against it (later tagged with a fluorescent dye) can be

produced.

4. Used widely in cytogenetics.

Electron Microscope

Basic Principle

The resolution of the light microscope has been shown

to be limited by the NA and the wavelength of light

employed. As the degree of correction in glass lenses is

very high, the main limitation is imposed by the light

(e.g. half wavelength of light), giving a normal resolution

of approximately 250 nm; and when UV light is used,

a resolution of about 100 nm. By the substitution of an

electron beam for light rays, a much greater degree of

resolution can be obtained; since at an acceleration of

50,000 volts, electrons have a wavelength of only 0.001 nm;

therefore, a theoretical resolving power of 0.0005 nm could

be attained, which would enable molecules to be seen.

Unfortunately, the degree of correction that is currently

feasible with transmission electron microscope (TEM)

lenses will permit a resolution of only 0.25 nm, but this is

still a thousand times greater than that possible with the

light microscope. A further difficulty with the TEM is that,

since electrons have poor penetrating power, the sections

to be examined must be very thin, less than 50 nm thick.

This necessitates the use of special hard embedding media

(plastics) and special ultra-microtomes to cut such thin

sections. Steel knives cannot be used to cut these sections;

either glass or diamond knives are used.

Weighing Scales or Analytical Balance

Weighing scales: For weighing large quantities.

Analytical balance: For accurate weighing of smaller

quantities.

Use and Care

1. The weighing equipment must be placed on a firm

bench, away from vibration, draughts, direct sunlight

and dust.

2. It should be kept perfectly horizontal by altering the

screws on which the equipment stands.

3. Chemicals, etc. should never be placed directly on the

pans. Weigh them in a container.

4. Never touch the weights with hands, handle them with

forceps.

5. The balance should be at rest before adding or

removing the weights or chemicals.

6. Before taking the reading, the glass window of the

instrument should be closed.

Electronic analytical balances are also available. Made

by various companies, these are very accurate.

Centrifuge

Centrifuge is used to sediment or deposit rapidly particles

such as cells which may be suspended in a fluid. The speed

is expressed as rpm, i.e. revolutions per minute.

Relative Centrifugal Force (RCF)

More important than rpm is relative centrifugal force

(RCF). RCF is expressed as the acceleration due to gravity

or G (dynes per cm). The formula is:

G = 0.00001118 × (r) × (n)2

where r = radius in centimeters

and n = revolutions per minute.

The time of centrifugation is equally important. The tubes

should be spun for a definite period to obtain the desired

effect.

Types of Centrifuge

Hand Centrifuge

Fixed to the bench, the handle is rotated manually. It gives

low speeds only.

FIG. 1.12: Components of fluorescence system

Laboratory 23

Motor-driven Centrifuge

Operated through mains electricity supply. The tubes

may be kept in a fixed angle head or in a swing out head

(Figs 1.13 and 1.14).

Microhematocrit Centrifuge

Also motor driven for finding out packed cell volume

(PCV) of red blood cells (RBCs). In this, blood-filled

capillary tubes are spun and later the percentage of

RBC-filled column is estimated (Figs 1.15 and 1.16).

Use and Care

1. Use centrifuge tubes made of strong glass and they

should not be too long.

2. The opposite tubes should be balanced properly.

3. The centrifuge speed should be increased gradually.

4. The instrument should be kept clean. If something

spills over inside, it should be cleaned and the

instrument disinfected, if necessary.

FIG. 1.13: Swing out head centrifuge

(Courtesy: Yorco Sales Pvt. Ltd)

FIG. 1.14: Motor driven centrifuge with rpm. indicator and auto

(timed) shut off

(Courtesy: Yorco Sales Pvt. Ltd)

FIG. 1.15: Dual centrifuge routine centrifuge with microhematocrit

attachment

(Courtesy: Yorco Sales Pvt. Ltd)

FIG. 1.16: Microhematocrit centrifuge and its parts

(Courtesy: Yorco Sales Pvt. Ltd)

24 Concise Book of Medical Laboratory Technology: Methods and Interpretations Glassware (Many Items are now Made of Plastic)

1. Flasks—are of different sizes and shapes.

a. Erlenmeyer or conical flasks—for heating and

boiling liquids (Fig. 1.17).

b. Volumetric flasks—are graduated for getting

exact volume of liquids (Fig. 1.18).

c. Round and flat-bottomed flasks for preparing

solutions (Figs 1.19A and B).

2. Beakers—available in different sizes (Fig. 1.20).

3. Bottles

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